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Mouse CD19 Recombinant antibody, PBS Only (Capture/Detector)

CD19 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240135A3

Cat no : 83254-1-PBS

Print datasheet

Synonyms

CD19 antigen, 240135A3

验证数据展示

  • WB analysis using 83254-1-RR (same clone as 83254-1-PBS)

    WB analysis using 83254-1-RR (same clone as 83254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83254-1-RR (CD19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 83254-1-RR (same clone as 83254-1-PBS)

    IHC staining of rat spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00253-1

    Cytometric bead array standard curve of MP00253-1

    Cytometric bead array standard curve of MP00253-1, Mouse CD19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83254-2-PBS. Detection antibody: 83254-1-PBS. Standard: Eg0907. Range: 2.5-80 ng/mL.

  • Cytometric bead array standard curve of MP00253-3

    Cytometric bead array standard curve of MP00253-3

    Cytometric bead array standard curve of MP00253-3, Mouse CD19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83254-1-PBS. Detection antibody: 83254-4-PBS. Standard: Eg0907. Range: 5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83254-1-RR

    Affinity and Kinetic Characterization of 83254-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83254-1-RR against Mouse CD19 were performed. The affinity constant is 0.303 nM.

产品信息

83254-1-PBS targets CD19 as part of a matched antibody pair:

MP00253-1: 83254-2-PBS capture and 83254-1-PBS detection (validated in Cytometric bead array)

MP00253-3: 83254-1-PBS capture and 83254-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD19 antigen
Synonyms CD19 antigen, 240135A3
Calculated Molecular Weight60 kDa
Observed Molecular Weight62-70 kDa
GenBank Accession NumberNM_009844.2
Gene Symbol Cd19
Gene ID (NCBI) 12478
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25918
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD19 is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).