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验证数据展示

  • WB analysis using 83267-4-RR (same clone as 83267-4-PBS)

    WB analysis using 83267-4-RR (same clone as 83267-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83267-4-RR (MAEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00233-4

    Sandwich ELISA standard curve of MP00233-4

    Sandwich ELISA standard curve of MP00233-4, Human MAEA Recombinant Matched Antibody Pair - PBS only. 83267-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27150. 83267-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP00233-4

    Sample test of MP00233-4

    The mean MAEA concentration was determined to be 38.39 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

  • IF Staining of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

    IF Staining of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAEA antibody (83267-4-RR, Clone: 240097E6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

    FC experiment of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAEA Recombinant antibody (83267-4-RR, Clone:240097E6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00233-3

    Cytometric bead array standard curve of MP00233-3

    Cytometric bead array standard curve of MP00233-3, MAEA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83267-4-PBS. Detection antibody: 83267-2-PBS. Standard: Ag27150. Range: 2.5-100 ng/mL.

  • Affinity and Kinetic Characterization of 83267-4-PBS

    Affinity and Kinetic Characterization of 83267-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 83267-4-PBS against Human MAEA was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83267-4-RR

    Affinity and Kinetic Characterization of 83267-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83267-4-RR against Human MAEA were performed. The affinity constant is 0.283 nM.

MAEA Recombinant antibody, PBS Only (Capture/Detector)

MAEA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83267-4-PBS

产品说明书

CloneNo. 240097E6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

EMP, EMLP, EC:2.3.2.27, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MAEA Ready-To-Use IHC Kit. MAEA primary antibody included.

产品信息

83267-4-PBS targets MAEA as part of a matched antibody pair:

MP00233-3: 83267-4-PBS capture and 83267-2-PBS detection (validated in Cytometric bead array)

MP00233-4: 83267-3-PBS capture and 83267-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 MAEA fusion protein Ag27150 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 macrophage erythroblast attacher
别名 EMP, EMLP, EC:2.3.2.27, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein
计算分子量 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC001225
基因名称 MAEA
Gene ID (NCBI) 10296
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7L5Y9
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).