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EPB41L3 Recombinant antibody

EPB41L3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230501D7

Cat no : 83275-2-RR

Print datasheet

Synonyms

DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3, 4.1B

验证数据展示

  • FC experiment of U2OS using 83275-2-RR

    FC experiment of U2OS using 83275-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug EPB41L3 Recombinant antibody (83275-2-RR, Clone:230501D7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of U2OS using 83275-2-RR

    WB analysis of U2OS using 83275-2-RR

    U2OS cells were subjected to SDS PAGE followed by western blot with 83275-2-RR (EPB41L3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83275-2-RR

    Affinity and Kinetic Characterization of 83275-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83275-2-RR against Human EPB41L3 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy EPB41L3 Ready-To-Use IHC Kit. EPB41L3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83275-2-RR is EPB41L3 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen EPB41L3 fusion protein Ag1089 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name erythrocyte membrane protein band 4.1-like 3
Synonyms DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3, 4.1B
Calculated Molecular Weight 121 kDa
Observed Molecular Weight120-130 kDa
GenBank Accession NumberBC008377
Gene Symbol EPB41L3
Gene ID (NCBI) 23136
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y2J2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPB41L3, also known as 4.1B and DAL1, belongs to the protein 4.1 family. It is a tumor suppressor that inhibits cell proliferation and promotes apoptosis. EPB41L3 modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5 (PMID: 15334060, 15737618). DAL1 loss is an early event in meningioma tumorigenesis (PMID: 10888600).

实验方案

Product Specific Protocols
WB protocol for EPB41L3 antibody 83275-2-RRDownload protocol
FC protocol for EPB41L3 antibody 83275-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols