• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

    WB analysis of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 83275-2-RR (EPB41L3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83275-2-PBS in a different storage buffer formulation.

  • WB analysis of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

    WB analysis of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

    WB result of EPB41L3 antibody (83275-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPB41L3 transfected U2OS cells. This data was developed using the same antibody clone with 83275-2-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

    FC experiment of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug EPB41L3 Recombinant antibody (83275-2-RR, Clone:230501D7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83275-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83275-2-RR

    Affinity and Kinetic Characterization of 83275-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83275-2-RR against Human EPB41L3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

EPB41L3 Recombinant antibody, PBS Only

EPB41L3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83275-2-PBS

产品说明书

CloneNo. 230501D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3, 4.1B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy EPB41L3 Ready-To-Use IHC Kit. EPB41L3 primary antibody included.

产品信息

83275-2-PBS targets EPB41L3 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 EPB41L3 fusion protein Ag1089 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 erythrocyte membrane protein band 4.1-like 3
别名 DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3, 4.1B
计算分子量 121 kDa
观测分子量120-130 kDa
GenBank蛋白编号BC008377
基因名称 EPB41L3
Gene ID (NCBI) 23136
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y2J2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPB41L3, also known as 4.1B and DAL1, belongs to the protein 4.1 family. It is a tumor suppressor that inhibits cell proliferation and promotes apoptosis. EPB41L3 modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5 (PMID: 15334060, 15737618). DAL1 loss is an early event in meningioma tumorigenesis (PMID: 10888600).