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EPB41L3 Recombinant antibody, PBS Only

EPB41L3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230501D7

Cat no : 83275-2-PBS

Print datasheet

Synonyms

DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3, 4.1B

验证数据展示

  • WB analysis of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

    WB analysis of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 83275-2-RR (EPB41L3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83275-2-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

    FC experiment of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug EPB41L3 Recombinant antibody (83275-2-RR, Clone:230501D7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83275-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83275-2-RR

    Affinity and Kinetic Characterization of 83275-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83275-2-RR against Human EPB41L3 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy EPB41L3 Ready-To-Use IHC Kit. EPB41L3 primary antibody included.

产品信息

83275-2-PBS targets EPB41L3 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen EPB41L3 fusion protein Ag1089 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name erythrocyte membrane protein band 4.1-like 3
Synonyms DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3, 4.1B
Calculated Molecular Weight 121 kDa
Observed Molecular Weight120-130 kDa
GenBank Accession NumberBC008377
Gene Symbol EPB41L3
Gene ID (NCBI) 23136
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y2J2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPB41L3, also known as 4.1B and DAL1, belongs to the protein 4.1 family. It is a tumor suppressor that inhibits cell proliferation and promotes apoptosis. EPB41L3 modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5 (PMID: 15334060, 15737618). DAL1 loss is an early event in meningioma tumorigenesis (PMID: 10888600).