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Conjugate
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EPB41L3 Recombinant antibody

EPB41L3 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230501D7

Cat no : 83275-2-RR

Print datasheet

Synonyms

DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3, 4.1B

验证数据展示

  • FC experiment of U2OS using 83275-2-RR

    FC experiment of U2OS using 83275-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug EPB41L3 Recombinant antibody (83275-2-RR, Clone:230501D7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of U2OS using 83275-2-RR

    WB analysis of U2OS using 83275-2-RR

    U2OS cells were subjected to SDS PAGE followed by western blot with 83275-2-RR (EPB41L3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83275-2-RR

    Affinity and Kinetic Characterization of 83275-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83275-2-RR against Human EPB41L3 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy EPB41L3 Ready-To-Use IHC Kit. EPB41L3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83275-2-RR targets EPB41L3 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen EPB41L3 fusion protein Ag1089 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name erythrocyte membrane protein band 4.1-like 3
Synonyms DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3, 4.1B
Calculated Molecular Weight 121 kDa
Observed Molecular Weight120-130 kDa
GenBank Accession NumberBC008377
Gene Symbol EPB41L3
Gene ID (NCBI) 23136
RRIDAB_3670945
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y2J2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPB41L3, also known as 4.1B and DAL1, belongs to the protein 4.1 family. It is a tumor suppressor that inhibits cell proliferation and promotes apoptosis. EPB41L3 modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5 (PMID: 15334060, 15737618). DAL1 loss is an early event in meningioma tumorigenesis (PMID: 10888600).

实验方案

Product Specific Protocols
WB protocol for EPB41L3 antibody 83275-2-RRDownload protocol
FC protocol for EPB41L3 antibody 83275-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols