验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, A549 cells, Jurkat cells, HeLa cells, HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83296-4-RR targets FGD6 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | FGD6 fusion protein Ag22211 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | FYVE, RhoGEF and PH domain containing 6 |
| 别名 | Zinc finger FYVE domain-containing protein 24, ZFYVE24, FYVE, RhoGEF and PH domain-containing protein 6, 240260A6 |
| 计算分子量 | 1430 aa, 161 kDa |
| 观测分子量 | 161 kDa |
| GenBank蛋白编号 | BC013319 |
| 基因名称 | FGD6 |
| Gene ID (NCBI) | 55785 |
| RRID | AB_3670963 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q6ZV73 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FGD6, also known as ZFYVE24, is localized on the Homo sapiens chromosome 12q22 and consists of 1,400 amino acids. Previous studies have reported that variations in FGD6 increase individual susceptibility to polypoidal choroidal vasculopathy. It was also demonstrated that FGD6 coordinates cell polarity and endosomal membrane recycling in osteoclasts by regulating the assembly of different actin-based protein networks and activating Cdc42 at different locations.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FGD6 antibody 83296-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |