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验证数据展示

  • WB analysis using 83360-2-RR

    WB analysis using 83360-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83360-2-RR (SLC25A46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of Caco-2 using 83360-2-RR

    FC experiment of Caco-2 using 83360-2-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC25A46 Recombinant antibody (83360-2-RR, Clone:240114G3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83360-2-RR

    Affinity and Kinetic Characterization of 83360-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83360-2-RR against Human SLC25A46 were performed. The affinity constant is 0.879 nM.

SLC25A46 Recombinant antibody

SLC25A46 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83360-2-RR

产品说明书

CloneNo. 240114G3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

TB1, Mitochondrial outer membrane protein SLC25A46, 240114G3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells, HeLa cells, Jurkat cells, MDA-MB-231 cells, mouse brain tissue
Positive FC (Intra) detected inCaco-2 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83360-2-RR targets SLC25A46 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 SLC25A46 fusion protein Ag27293 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 25, member 46
别名 TB1, Mitochondrial outer membrane protein SLC25A46, 240114G3
计算分子量 418 aa, 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC017169
基因名称 SLC25A46
Gene ID (NCBI) 91137
RRIDAB_3671014
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96AG3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Solute carrier family 25 member 46 (SLC25A46), a highly conserved protein among various species including vertebrates, is a nuclear gene encoding mitochondrial carriers. SLC25A46 is involved in mitochondrial fission and the transport of lipids from the endoplasmic reticulum to mitochondria (PMID: 33277645). SLC25A46 has 3 isoforms with molecular weights of 30, 37 and 46 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for SLC25A46 antibody 83360-2-RRDownload protocol
FC protocol for SLC25A46 antibody 83360-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols