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  • Featured Product
  • KD/KO Validated

KIAA1217 Recombinant antibody, PBS Only (Capture)

KIAA1217 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240276F3

Cat no : 83361-2-PBS

Print datasheet

Synonyms

SKT, Sickle tail protein homolog, 240276F3

验证数据展示

  • WB analysis using 83361-2-RR (same clone as 83361-2-PBS)

    WB analysis using 83361-2-RR (same clone as 83361-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83361-2-RR (KIAA1217 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83361-2-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 83361-2-RR (same clone as 83361-2-PBS)

    WB analysis of HEK-293 using 83361-2-RR (same clone as 83361-2-PBS)

    WB result of KIAA1217 antibody (83361-2-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIAA1217 transfected HEK-293 cells. This data was developed using the same antibody clone with 83361-2-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83361-2-RR (same clone as 83361-2-PBS)

    FC experiment of MCF-7 using 83361-2-RR (same clone as 83361-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug KIAA1217 Recombinant antibody (83361-2-RR, Clone:240276F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83361-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00371-1

    Cytometric bead array standard curve of MP00371-1

    Cytometric bead array standard curve of MP00371-1, KIAA1217 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83361-2-PBS. Detection antibody: 83361-1-PBS. Standard: Ag19367. Range: 0.78-100ng/mL.

  • Affinity and Kinetic Characterization of 83361-2-RR

    Affinity and Kinetic Characterization of 83361-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83361-2-RR against Human KIAA1217 were performed. The affinity constant is 1.46 nM.

产品信息

83361-2-PBS targets KIAA1217 as part of a matched antibody pair:

MP00371-1: 83361-2-PBS capture and 83361-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen KIAA1217 fusion protein Ag19367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name KIAA1217
Synonyms SKT, Sickle tail protein homolog, 240276F3
Calculated Molecular Weight 1943 aa, 214 kDa
Observed Molecular Weight100-110, 140-160, 210
GenBank Accession NumberBC018764
Gene Symbol KIAA1217
Gene ID (NCBI) 56243
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5T5P2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KIAA1217, sickle tail protein homolog, is required for normal development of intervertebral disks. It promotes epithelial-mesenchymal transition and hepatocellular carcinoma metastasis by interacting with and activating STAT3, indicating its potential as a therapeutic target for curbing HCC metastasis (PMID: 35008530). It is also a novel candidate gene associated with isolated and syndromic vertebral malformations (PMID: 32369272). KIAA1217 has 9 alternatively spliced isoforms of 100-110 kDa, 140-160 kDa, 210 kDa.