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MCP-1/CCL2 Recombinant antibody

MCP-1/CCL2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240341G3

Cat no : 83370-2-RR

Print datasheet

Synonyms

Ccl2, chemokine (C C motif) ligand 2, CCL-2, CCL 2, C-C motif chemokine 2

验证数据展示

  • WB analysis of NR8383 using 83370-2-RR

    WB analysis of NR8383 using 83370-2-RR

    Untreated, LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 83370-2-RR (Ccl2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83370-2-RR

    Affinity and Kinetic Characterization of 83370-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83370-2-RR against Rat MCP-1 were performed. The affinity constant is 0.836 nM.

经过测试的应用

Positive WB detected inLPS and Brefeldin A treated NR8383 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83370-2-RR is MCP-1/CCL2 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity rat
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 2
Synonyms Ccl2, chemokine (C C motif) ligand 2, CCL-2, CCL 2, C-C motif chemokine 2
Calculated Molecular Weight16 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberNM_031530
Gene Symbol MCP-1/CCL2
Gene ID (NCBI) 24770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14844
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

实验方案

Product Specific Protocols
WB protocol for MCP-1/CCL2 antibody 83370-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols