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CMIP Recombinant antibody

CMIP Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240059C12

Cat no : 83381-1-RR

Print datasheet

Synonyms

Tc Mip, c-Mip, C-Maf-inducing protein, c Mip, c Maf inducing protein

验证数据展示

  • WB analysis of human peripheral blood platelets using 83381-1-RR

    WB analysis of human peripheral blood platelets using 83381-1-RR

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 83381-1-RR (CMIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83381-1-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 83381-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83381-1-RR (CMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CMIP antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 83381-1-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 83381-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83381-1-RR (CMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CMIP antibody included
  • FC experiment of A549 using 83381-1-RR

    FC experiment of A549 using 83381-1-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug CMIP Recombinant Antibody (83381-1-RR, Clone:240059C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive WB detected inhuman peripheral blood platelets
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy CMIP Ready-To-Use IHC Kit. CMIP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83381-1-RR targets CMIP in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen CMIP fusion protein Ag3475 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name c-Maf-inducing protein
Synonyms Tc Mip, c-Mip, C-Maf-inducing protein, c Mip, c Maf inducing protein
Calculated Molecular Weight 86 kDa
Observed Molecular Weight69 kDa
GenBank Accession NumberBC038113
Gene Symbol CMIP
Gene ID (NCBI) 80790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IY22
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The gene of CMIP was isolated from one patient of minimal change nephrotic syndrome. The gene encodes two isoforms: CMIP and truncated CMIP. The predicted CMIP and truncated CMIP proteins contain 739 and 679 amino acids and have calculated molecular masses of 85 and 75 kD, respectively. The two isoforms have different distribution and expression pattern.

实验方案

Product Specific Protocols
WB protocol for CMIP antibody 83381-1-RRDownload protocol
IHC protocol for CMIP antibody 83381-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols