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CD45 Recombinant antibody

CD45 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240356G10

Cat no : 83396-5-RR

Print datasheet

Synonyms

Ptprc, Leukocyte common antigen, LCA, B220, 240356G10

验证数据展示

  • WB analysis using 83396-5-RR

    WB analysis using 83396-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83396-5-RR (CD45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 83396-5-RR

    IF Staining of RAW 264.7 using 83396-5-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IF Staining of RAW 264.7 using 83396-5-RR

    IF Staining of RAW 264.7 using 83396-5-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity and Kinetic Characterization of 83396-5-RR

    Affinity and Kinetic Characterization of 83396-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83396-5-RR against Mouse CD45 were performed. The affinity constant is 0.415 nM.

经过测试的应用

Positive WB detected inJ774A.1 cells, RAW 264.7 cells
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83396-5-RR is CD45 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms Ptprc, Leukocyte common antigen, LCA, B220, 240356G10
Calculated Molecular Weight145 kDa
Observed Molecular Weight190 kDa
GenBank Accession NumberNM_001111316.2
Gene Symbol CD45
Gene ID (NCBI) 19264
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP06800-4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
WB protocol for CD45 antibody 83396-5-RRDownload protocol
IF protocol for CD45 antibody 83396-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols