验证数据展示
经过测试的应用
| Positive IF/ICC detected in | RAW 264.7 cells |
For other applications, we recommend the unconjugated version of this antibody, 83396-5-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83396-5 targets CD45 in IF/ICC applications and shows reactivity with mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | mouse |
| 免疫原 | Fusion Protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, C |
| 别名 | Ptprc, Leukocyte common antigen, LCA, B220, 240356G10 |
| 计算分子量 | 145 kDa |
| 观测分子量 | 190 kDa |
| GenBank蛋白编号 | NM_001111316.2 |
| 基因名称 | CD45 |
| Gene ID (NCBI) | 19264 |
| RRID | AB_3673248 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P06800-4 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD45 antibody CL488-83396-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |