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PLK2 Recombinant antibody

PLK2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230520B2

Cat no : 83431-1-RR

Print datasheet

Synonyms

PLK-2, PLK 2, hSNK, hPlk2, EC:2.7.11.21

验证数据展示

  • IF Staining of hTERT-RPE1 using 83431-1-RR

    IF Staining of hTERT-RPE1 using 83431-1-RR

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using PLK2 antibody (83431-1-RR, Clone: 230520B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • FC experiment of A549 using 83431-1-RR

    FC experiment of A549 using 83431-1-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Plk2 Recombinant Antibody (83431-1-RR, Clone:230520B2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83431-1-RR

    Affinity and Kinetic Characterization of 83431-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83431-1-RR against Human PLK2 were performed. The affinity constant is 1.53 nM.

经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83431-1-RR is PLK2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen PLK2 fusion protein Ag29837 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name polo-like kinase 2 (Drosophila)
Synonyms PLK-2, PLK 2, hSNK, hPlk2, EC:2.7.11.21
Calculated Molecular Weight 685 aa, 78 kDa
GenBank Accession NumberBC013879
Gene Symbol PLK2
Gene ID (NCBI) 10769
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NYY3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLK2(Polo-like kinase 2) is also named as SNK and belongs to the Ser/Thr protein kinase family. It plays a role in normal cell division. Plk2 expression is detected only in G1, and endogenous Plk2 is rapidly turned over. Therefore, the expression of the three mammalian Plks during the cell cycle resembles the successive transition of the cyclins, prompting speculation that Plks may reg-ulate the cell cycle in a manner analogous to that of Cdks(PMID:12972611).

实验方案

Product Specific Protocols
IF protocol for PLK2 antibody 83431-1-RRDownload protocol
FC protocol for PLK2 antibody 83431-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols