验证数据展示
经过测试的应用
| Positive IF/ICC detected in | hTERT-RPE1 cells |
For other applications, we recommend the unconjugated version of this antibody, 83431-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83431 targets PLK2 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | PLK2 fusion protein Ag29837 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | polo-like kinase 2 (Drosophila) |
| 别名 | |
| 计算分子量 | 685 aa, 78 kDa |
| GenBank蛋白编号 | BC013879 |
| 基因名称 | PLK2 |
| Gene ID (NCBI) | 10769 |
| RRID | AB_3673252 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NYY3 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLK2(Polo-like kinase 2) is also named as SNK and belongs to the Ser/Thr protein kinase family. It plays a role in normal cell division. Plk2 expression is detected only in G1, and endogenous Plk2 is rapidly turned over. Therefore, the expression of the three mammalian Plks during the cell cycle resembles the successive transition of the cyclins, prompting speculation that Plks may reg-ulate the cell cycle in a manner analogous to that of Cdks(PMID:12972611).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PLK2 antibody CL488-83431 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |