CoraLite® Plus 488-conjugated PLK2 Recombinant antibody
PLK2 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
230520B2
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | hTERT-RPE1 cells |
For other applications, we recommend the unconjugated version of this antibody, 83431-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-83431 is PLK2 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | PLK2 fusion protein Ag29837 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | polo-like kinase 2 (Drosophila) |
| Synonyms | |
| Calculated Molecular Weight | 685 aa, 78 kDa |
| GenBank Accession Number | BC013879 |
| Gene Symbol | PLK2 |
| Gene ID (NCBI) | 10769 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NYY3 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLK2(Polo-like kinase 2) is also named as SNK and belongs to the Ser/Thr protein kinase family. It plays a role in normal cell division. Plk2 expression is detected only in G1, and endogenous Plk2 is rapidly turned over. Therefore, the expression of the three mammalian Plks during the cell cycle resembles the successive transition of the cyclins, prompting speculation that Plks may reg-ulate the cell cycle in a manner analogous to that of Cdks(PMID:12972611).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PLK2 antibody CL488-83431 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |