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CD5 Recombinant antibody

CD5 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240244C2

Cat no : 83451-4-RR

Print datasheet

Synonyms

Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen

验证数据展示

  • WB analysis of EL-4 using 83451-4-RR

    WB analysis of EL-4 using 83451-4-RR

    EL-4 cells were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 83451-4-RR

    IHC staining of mouse spleen using 83451-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83451-4-RR (CD5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 83451-4-RR

    IHC staining of mouse spleen using 83451-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83451-4-RR (CD5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83451-4-RR

    Affinity and Kinetic Characterization of 83451-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83451-4-RR against Mouse CD5 were performed. The affinity constant is 89.9 pM.

经过测试的应用

Positive WB detected inEL-4 cells, mouse thymus tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83451-4-RR is CD5 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD5 antigen
Synonyms Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen
Calculated Molecular Weight54 kDa
Observed Molecular Weight 55 kDa, 67 kDa
GenBank Accession NumberNM_007650.3
Gene Symbol Cd5
Gene ID (NCBI) 12507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP13379
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
WB protocol for CD5 antibody 83451-4-RRDownload protocol
IHC protocol for CD5 antibody 83451-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols