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验证数据展示

  • WB analysis using 83468-6-RR

    WB analysis using 83468-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83468-6-RR (ELAC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 83468-6-RR

    FC experiment of A431 using 83468-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ELAC2 Recombinant antibody (83468-6-RR, Clone:240487B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83468-6-RR

    Affinity and Kinetic Characterization of 83468-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83468-6-RR against Human ELAC2 were performed. The affinity constant is below 1 pM.

ELAC2 Recombinant antibody

ELAC2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83468-6-RR

产品说明书

CloneNo. 240487B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

FLJ10530, ELC2, ElaC homolog protein 2, elaC homolog 2 (E. coli), EC:3.1.26.11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, Raji cells, DU 145 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy ELAC2 Ready-To-Use IHC Kit. ELAC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83468-6-RR targets ELAC2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 ELAC2 fusion protein Ag0105 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 elaC homolog 2 (E. coli)
别名 FLJ10530, ELC2, ElaC homolog protein 2, elaC homolog 2 (E. coli), EC:3.1.26.11
计算分子量 92 kDa
观测分子量 97 kDa
GenBank蛋白编号BC004158
基因名称 ELAC2
Gene ID (NCBI) 60528
RRIDAB_3671098
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9BQ52
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Putative prostate cancer susceptibility protein (ELAC2) is encoded by HPC2/ELAC2 gene. HPC2 is a potential prostate cancer predisposition gene playing a role in genetic susceptibility to prostate cancer.It is a zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity and probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.Defects in ELAC2 may be a cause of susceptibility to prostate cancer (PC).

实验方案

Product Specific Protocols
WB protocol for ELAC2 antibody 83468-6-RRDownload protocol
FC protocol for ELAC2 antibody 83468-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols