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TRIM22 Recombinant antibody

TRIM22 Uni-rAbTM Recombinant Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

240411D6

Cat no : 83479-4-RR

Print datasheet

Synonyms

RING-type E3 ubiquitin transferase TRIM22, GPSTAF50, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM22, 50 kDa-stimulated trans-acting factor

验证数据展示

  • WB analysis using 83479-4-RR

    WB analysis using 83479-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83479-4-RR (TRIM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83479-4-RR

    WB analysis using 83479-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83479-4-RR (TRIM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 83479-4-RR

    FC experiment of U2OS using 83479-4-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug TRIM22 Recombinant antibody (83479-4-RR, Clone:240411D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83479-4-RR

    Affinity and Kinetic Characterization of 83479-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83479-4-RR against Human TRIM22 were performed. The affinity constant is 0.181 nM.

经过测试的应用

Positive WB detected inHeLa cells, HL-60 cells, K-562 cells, A549 cells, U2OS cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy TRIM22 Ready-To-Use IHC Kit. TRIM22 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83479-4-RR is TRIM22 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA, FC (Intra) Application Description
Tested Reactivity Human
Immunogen TRIM22 fusion protein Ag4683 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tripartite motif-containing 22
Synonyms RING-type E3 ubiquitin transferase TRIM22, GPSTAF50, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM22, 50 kDa-stimulated trans-acting factor
Calculated Molecular Weight 498 aa, 57 kDa
Observed Molecular Weight57 kDa
GenBank Accession NumberBC035582
Gene Symbol TRIM22
Gene ID (NCBI) 10346
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IYM9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tripartite motif containing 22 (TRIM22), a member of the TRIM/RBCC family, has been reported to activate the nuclear factor-kappa B (NF-κB) pathway in unstimulated macrophage cell lines (PMID: 23818111). TRIM22 is involved in the host antiviral response, TRIM22 expression is modulated in response to several other viruses and viral antigens.TRIM22 expression is high in monocytes and early granulocytes but decreases in the lymphocyte and late granulocyte populations and is undetectable in erythroid

实验方案

Product Specific Protocols
WB protocol for TRIM22 antibody 83479-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols