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TRIM22 Recombinant antibody, PBS Only

TRIM22 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240411D6

Cat no : 83479-4-PBS

Print datasheet

Synonyms

RING-type E3 ubiquitin transferase TRIM22, GPSTAF50, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM22, 50 kDa-stimulated trans-acting factor

验证数据展示

  • WB analysis using 83479-4-RR (same clone as 83479-4-PBS)

    WB analysis using 83479-4-RR (same clone as 83479-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83479-4-RR (TRIM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83479-4-PBS in a different storage buffer formulation.

  • WB analysis using 83479-4-RR (same clone as 83479-4-PBS)

    WB analysis using 83479-4-RR (same clone as 83479-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83479-4-RR (TRIM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83479-4-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83479-4-RR (same clone as 83479-4-PBS)

    FC experiment of U2OS using 83479-4-RR (same clone as 83479-4-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug TRIM22 Recombinant antibody (83479-4-RR, Clone:240411D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83479-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83479-4-RR

    Affinity and Kinetic Characterization of 83479-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83479-4-RR against Human TRIM22 were performed. The affinity constant is 0.181 nM.

Planning an IHC experiment? We recommend our IHCeasy TRIM22 Ready-To-Use IHC Kit. TRIM22 primary antibody included.

产品信息

The immunogen of 83479-4-PBS is TRIM22 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen TRIM22 fusion protein Ag4683 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tripartite motif-containing 22
Synonyms RING-type E3 ubiquitin transferase TRIM22, GPSTAF50, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM22, 50 kDa-stimulated trans-acting factor
Calculated Molecular Weight 498 aa, 57 kDa
GenBank Accession NumberBC035582
Gene Symbol TRIM22
Gene ID (NCBI) 10346
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IYM9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C