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验证数据展示

  • WB analysis using 83479-4-RR

    WB analysis using 83479-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83479-4-RR (TRIM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 83479-4-RR

    IF Staining of A431 using 83479-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TRIM22 antibody (83479-4-RR, Clone: 240411D6 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • WB analysis using 83479-4-RR

    WB analysis using 83479-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83479-4-RR (TRIM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 83479-4-RR

    FC experiment of U2OS using 83479-4-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug TRIM22 Recombinant antibody (83479-4-RR, Clone:240411D6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83479-4-RR

    Affinity and Kinetic Characterization of 83479-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83479-4-RR against Human TRIM22 were performed. The affinity constant is 0.181 nM.

TRIM22 Recombinant antibody

TRIM22 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83479-4-RR

产品说明书

CloneNo. 240411D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

RING-type E3 ubiquitin transferase TRIM22, GPSTAF50, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM22, 50 kDa-stimulated trans-acting factor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HL-60 cells, K-562 cells, A549 cells, U2OS cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy TRIM22 Ready-To-Use IHC Kit. TRIM22 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83479-4-RR targets TRIM22 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 TRIM22 fusion protein Ag4683 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 22
别名 RING-type E3 ubiquitin transferase TRIM22, GPSTAF50, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM22, 50 kDa-stimulated trans-acting factor
计算分子量 498 aa, 57 kDa
观测分子量57 kDa
GenBank蛋白编号BC035582
基因名称 TRIM22
Gene ID (NCBI) 10346
RRIDAB_3671107
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8IYM9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tripartite motif containing 22 (TRIM22), a member of the TRIM/RBCC family, has been reported to activate the nuclear factor-kappa B (NF-κB) pathway in unstimulated macrophage cell lines (PMID: 23818111). TRIM22 is involved in the host antiviral response, TRIM22 expression is modulated in response to several other viruses and viral antigens.TRIM22 expression is high in monocytes and early granulocytes but decreases in the lymphocyte and late granulocyte populations and is undetectable in erythroid

实验方案

Product Specific Protocols
WB protocol for TRIM22 antibody 83479-4-RRDownload protocol
IF protocol for TRIM22 antibody 83479-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols