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STEAP3 Recombinant antibody

STEAP3 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1N5

Cat no : 83484-2-RR

Print datasheet

Synonyms

hpHyde, EC:1.16.1.-, Dudulin-2, Dudulin 2, dudlin 2

验证数据展示

  • WB analysis using 83484-2-RR

    WB analysis using 83484-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83484-2-RR (STEAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83484-2-RR

    FC experiment of HeLa using 83484-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug STEAP3 Recombinant antibody (83484-2-RR, Clone:1N5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83484-2-RR

    Affinity and Kinetic Characterization of 83484-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83484-2-RR against Human STEAP3 were performed. The affinity constant is 0.89 nM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, PC-12 cells, C6 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STEAP3 Ready-To-Use IHC Kit. STEAP3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83484-2-RR targets STEAP3 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen STEAP3 fusion protein Ag29528 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name STEAP family member 3
Synonyms hpHyde, EC:1.16.1.-, Dudulin-2, Dudulin 2, dudlin 2
Calculated Molecular Weight 488 aa, 55 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC042150
Gene Symbol STEAP3
Gene ID (NCBI) 55240
RRIDAB_3671112
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ658P3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STEAP3 (Six-Transmembrane Epithelial Antigen of Prostate 3) is also named as TSAP6, Dudulin-2 and pHyde, and belongs to the STEAP family. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).

实验方案

Product Specific Protocols
WB protocol for STEAP3 antibody 83484-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols