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验证数据展示

  • WB analysis of Raji using 83507-2-RR

    WB analysis of Raji using 83507-2-RR

    Raji cells were subjected to SDS PAGE followed by western blot with 83507-2-RR (ZC3H12D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83507-2-RR

    FC experiment of HeLa using 83507-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ZC3H12D Recombinant antibody (83507-2-RR, Clone:240464C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83507-2-RR

    Affinity and Kinetic Characterization of 83507-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83507-2-RR against Human ZC3H12D were performed. The affinity constant is 0.343 nM.

ZC3H12D Recombinant antibody

ZC3H12D Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83507-2-RR

产品说明书

CloneNo. 240464C5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

MCP-induced protein 4, MCP induced protein 4, FLJ46041, EC:3.1.-.-, C6orf95

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ZC3H12D Ready-To-Use IHC Kit. ZC3H12D primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83507-2-RR targets ZC3H12D in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 ZC3H12D fusion protein Ag21770 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger CCCH-type containing 12D
别名 MCP-induced protein 4, MCP induced protein 4, FLJ46041, EC:3.1.-.-, C6orf95
计算分子量 527 aa, 58 kDa
观测分子量 55-58 kDa
GenBank蛋白编号BC157832
基因名称 ZC3H12D
Gene ID (NCBI) 340152
RRIDAB_3671132
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDA2A288
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZC3H12D, also named as MCP induced protein 4, is a 527 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12D exists as three isoforms and localizes in the cytoplasm. ZC3H12D may regulate cell growth likely by suppressing RB1 phosphorylation and serves as a tumor suppressor in certain leukemia cells. ZC3H12D is expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines.

实验方案

Product Specific Protocols
WB protocol for ZC3H12D antibody 83507-2-RRDownload protocol
FC protocol for ZC3H12D antibody 83507-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols