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ZC3H12D Recombinant antibody, PBS Only (Detector)

ZC3H12D Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240464C5

Cat no : 83507-2-PBS

Print datasheet

Synonyms

MCP-induced protein 4, MCP induced protein 4, FLJ46041, EC:3.1.-.-, C6orf95

验证数据展示

  • WB analysis of Raji using 83507-2-RR (same clone as 83507-2-PBS)

    WB analysis of Raji using 83507-2-RR (same clone as 83507-2-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 83507-2-RR (ZC3H12D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83507-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83507-2-RR (same clone as 83507-2-PBS)

    FC experiment of HeLa using 83507-2-RR (same clone as 83507-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ZC3H12D Recombinant antibody (83507-2-RR, Clone:240464C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83507-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00495-1

    Cytometric bead array standard curve of MP00495-1

    Cytometric bead array standard curve of MP00495-1, ZC3H12D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83507-1-PBS. Detection antibody: 83507-2-PBS. Standard: Ag21770. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 83507-2-RR

    Affinity and Kinetic Characterization of 83507-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83507-2-RR against Human ZC3H12D were performed. The affinity constant is 0.343 nM.

Planning an IHC experiment? We recommend our IHCeasy ZC3H12D Ready-To-Use IHC Kit. ZC3H12D primary antibody included.

产品信息

83507-2-PBS targets ZC3H12D as part of a matched antibody pair:

MP00495-1: 83507-1-PBS capture and 83507-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ZC3H12D fusion protein Ag21770 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name zinc finger CCCH-type containing 12D
Synonyms MCP-induced protein 4, MCP induced protein 4, FLJ46041, EC:3.1.-.-, C6orf95
Calculated Molecular Weight 527 aa, 58 kDa
GenBank Accession NumberBC157832
Gene Symbol ZC3H12D
Gene ID (NCBI) 340152
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDA2A288
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C