SOD2 Recombinant antibody
SOD2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240462H8
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HUVEC cells |
Positive FC (Intra) detected in | A549 cells, HL-60 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83519-4-RR is SOD2 Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | SOD2 fusion protein Ag21388 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | superoxide dismutase 2, mitochondrial |
Synonyms | SODM, MNSOD, Mn SOD, IPOB, IPO B |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 25 kDa |
GenBank Accession Number | BC016934 |
Gene Symbol | SOD2 |
Gene ID (NCBI) | 6648 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P04179 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SOD2 antibody 83519-4-RR | Download protocol |
IHC protocol for SOD2 antibody 83519-4-RR | Download protocol |
IF protocol for SOD2 antibody 83519-4-RR | Download protocol |
FC protocol for SOD2 antibody 83519-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |