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SOD2 Recombinant antibody, PBS Only

SOD2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240462H8

Cat no : 83519-4-PBS

Print datasheet

Synonyms

SODM, MNSOD, Mn SOD, IPOB, IPO B

验证数据展示

  • WB analysis using 83519-4-RR (same clone as 83519-4-PBS)

    WB analysis using 83519-4-RR (same clone as 83519-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83519-4-RR (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83519-4-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83519-4-RR (same clone as 83519-4-PBS)

    IHC staining of human ovary cancer using 83519-4-RR (same clone as 83519-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83519-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SOD2 antibody included
  • IHC staining of human ovary cancer using 83519-4-RR (same clone as 83519-4-PBS)

    IHC staining of human ovary cancer using 83519-4-RR (same clone as 83519-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83519-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SOD2 antibody included
  • FC experiment of A549 using 83519-4-RR (same clone as 83519-4-PBS)

    FC experiment of A549 using 83519-4-RR (same clone as 83519-4-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Sod2 Recombinant Antibody (83519-4-RR, Clone:240462H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83519-4-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 83519-4-RR (same clone as 83519-4-PBS)

    IF Staining of HUVEC using 83519-4-RR (same clone as 83519-4-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (83519-4-RR, Clone: 240462H8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83519-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83519-4-RR

    Affinity and Kinetic Characterization of 83519-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83519-4-RR against Human SOD2 were performed. The affinity constant is 219 nM.

Planning an IHC experiment? We recommend our IHCeasy SOD2 Ready-To-Use IHC Kit. SOD2 primary antibody included.

产品信息

83519-4-PBS targets SOD2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SOD2 fusion protein Ag21388 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name superoxide dismutase 2, mitochondrial
Synonyms SODM, MNSOD, Mn SOD, IPOB, IPO B
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC016934
Gene Symbol SOD2
Gene ID (NCBI) 6648
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP04179
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.