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S100A9 Recombinant antibody

S100A9 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240539G9

Cat no : 83578-2-RR

Print datasheet

Synonyms

CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB

验证数据展示

  • WB analysis of MCF-7 using 83578-2-RR

    WB analysis of MCF-7 using 83578-2-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83578-2-RR

    WB analysis using 83578-2-RR

    HL-60 cells were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 83578-2-RR

    IF Staining of A549 using 83578-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of HeLa using 83578-2-RR

    IF Staining of HeLa using 83578-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 83578-2-RR

    Affinity and Kinetic Characterization of 83578-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83578-2-RR against Human S100A9 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inMCF-7 cells, HL-60 cells
Positive IF/ICC detected inA549 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy S100A9 Ready-To-Use IHC Kit. S100A9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83578-2-RR is S100A9 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen S100A9 fusion protein Ag25764 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name S100 calcium binding protein A9
Synonyms CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC047681
Gene Symbol S100A9
Gene ID (NCBI) 6280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06702
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

实验方案

Product Specific Protocols
WB protocol for S100A9 antibody 83578-2-RRDownload protocol
IF protocol for S100A9 antibody 83578-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols