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S100A9 Recombinant antibody, PBS Only (Capture)

S100A9 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240539G9

Cat no : 83578-2-PBS

Print datasheet

Synonyms

CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB

验证数据展示

  • WB analysis of MCF-7 using 83578-2-RR (same clone as 83578-2-PBS)

    WB analysis of MCF-7 using 83578-2-RR (same clone as 83578-2-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

  • WB analysis using 83578-2-PBS

    WB analysis using 83578-2-PBS

    Various lysates were subjected to SDS PAGE followed by western blot with 83578-2-PBS (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83578-2-RR (same clone as 83578-2-PBS)

    WB analysis using 83578-2-RR (same clone as 83578-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 83578-2-RR (same clone as 83578-2-PBS)

    IF Staining of A549 using 83578-2-RR (same clone as 83578-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83578-2-RR (same clone as 83578-2-PBS)

    IF Staining of HeLa using 83578-2-RR (same clone as 83578-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00569-3

    Cytometric bead array standard curve of MP00569-3

    Cytometric bead array standard curve of MP00569-3, S100A9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83578-2-PBS. Detection antibody: 83578-6-PBS. Standard: Ag25764. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83578-2-PBS

    Affinity and Kinetic Characterization of 83578-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83578-2-PBS against Human S100A9 was performed. The affinity constant is 0.389 nM.

  • Affinity and Kinetic Characterization of 83578-2-RR

    Affinity and Kinetic Characterization of 83578-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83578-2-RR against Human S100A9 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inTHP-1 cells, MCF-7 cells, HL-60 cells
Planning an IHC experiment? We recommend our IHCeasy S100A9 Ready-To-Use IHC Kit. S100A9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83578-2-PBS targets S100A9 as part of a matched antibody pair:

MP00569-3: 83578-2-PBS capture and 83578-6-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen S100A9 fusion protein Ag25764 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name S100 calcium binding protein A9
Synonyms CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC047681
Gene Symbol S100A9
Gene ID (NCBI) 6280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06702
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

实验方案

Product Specific Protocols
WB protocol for S100A9 antibody 83578-2-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols