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验证数据展示

  • WB analysis of MCF-7 using 83578-2-RR

    WB analysis of MCF-7 using 83578-2-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83578-2-RR

    WB analysis using 83578-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 83578-2-RR

    IF Staining of A549 using 83578-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of HeLa using 83578-2-RR

    IF Staining of HeLa using 83578-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 83578-2-RR

    Affinity and Kinetic Characterization of 83578-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83578-2-RR against Human S100A9 were performed. The affinity constant is below 1 pM.

S100A9 Recombinant antibody

S100A9 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83578-2-RR

产品说明书

CloneNo. 240539G9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, THP-1 cells, HL-60 cells
Positive IF/ICC detected inA549 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy S100A9 Ready-To-Use IHC Kit. S100A9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83578-2-RR targets S100A9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 S100A9 fusion protein Ag25764 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein A9
别名 CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB
计算分子量 13 kDa
观测分子量 14 kDa
GenBank蛋白编号BC047681
基因名称 S100A9
Gene ID (NCBI) 6280
RRIDAB_3671190
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06702
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

实验方案

Product Specific Protocols
WB protocol for S100A9 antibody 83578-2-RRDownload protocol
IF protocol for S100A9 antibody 83578-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols