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SEMA3A Recombinant antibody

SEMA3A Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240509E3

Cat no : 83579-5-RR

Print datasheet

Synonyms

SEMA1, Sema III, Hsema III, Hsema I, coll 1

验证数据展示

  • WB analysis using 83579-5-RR

    WB analysis using 83579-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83579-5-RR (SEMA3A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83579-5-RR

    Affinity and Kinetic Characterization of 83579-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83579-5-RR against Human SEMA3A were performed. The affinity constant is 0.228 nM.

经过测试的应用

Positive WB detected inSKOV-3 cells, PC-12 cells, C6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83579-5-RR is SEMA3A Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, rat
Immunogen SEMA3A fusion protein Ag27173 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Synonyms SEMA1, Sema III, Hsema III, Hsema I, coll 1
Calculated Molecular Weight 771 aa, 89 kDa
Observed Molecular Weight89 kDa
GenBank Accession NumberBC111416
Gene Symbol SEMA3A
Gene ID (NCBI) 10371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ14563
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEMA3A (Semaphorin 3A), a class-3 semaphorin signaling protein, is involved in cell signaling with PlexinA1 and Neuropilin-1 (NRP1) receptors and it is responsible for recruiting dendritic cells into lymphatics. It's widely expressed in different tissues (PMID:10766232). SEMA3A has been shown to exert osteoprotective effects, characterized by increasing bone formation while inhibiting the activation of osteoclast precursor cells (PMID:36598593). However, SEMA3A also modulates the immune system. SEMA3A expression in a myocardial infarct area enhances macrophage polarization and inhibits monocyte migration into the lesion area (PMID:28540528).

实验方案

Product Specific Protocols
WB protocol for SEMA3A antibody 83579-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols