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CCDC94 Recombinant antibody

CCDC94 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240517A1

Cat no : 83580-1-RR

Print datasheet

Synonyms

YJU2, Coiled-coil domain-containing protein 94, 240517A1

验证数据展示

  • WB analysis using 83580-1-RR

    WB analysis using 83580-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83580-1-RR (CCDC94 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 83580-1-RR

    IF Staining of A549 using 83580-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCDC94 antibody (83580-1-RR, Clone: 240517A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A549 using 83580-1-RR

    IF Staining of A549 using 83580-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCDC94 antibody (83580-1-RR, Clone: 240517A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 83580-1-RR

    FC experiment of A431 using 83580-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CCDC94 Recombinant antibody (83580-1-RR, Clone:240517A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83580-1-RR

    Affinity and Kinetic Characterization of 83580-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83580-1-RR against Human CCDC94 were performed. The affinity constant is 1.08 nM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cell, K-562 cells, HepG2 cells
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83580-1-RR is CCDC94 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen CCDC94 fusion protein Ag13764 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name coiled-coil domain containing 94
Synonyms YJU2, Coiled-coil domain-containing protein 94, 240517A1
Calculated Molecular Weight 323 aa, 37 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC000561
Gene Symbol CCDC94
Gene ID (NCBI) 55702
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BW85
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)

实验方案

Product Specific Protocols
WB protocol for CCDC94 antibody 83580-1-RRDownload protocol
IF protocol for CCDC94 antibody 83580-1-RRDownload protocol
FC protocol for CCDC94 antibody 83580-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols