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Conjugate
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验证数据展示

  • WB analysis using 83580-1-RR (same clone as 83580-1-PBS)

    WB analysis using 83580-1-RR (same clone as 83580-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83580-1-RR (CCDC94 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83580-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 83580-1-RR (same clone as 83580-1-PBS)

    IF Staining of A549 using 83580-1-RR (same clone as 83580-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCDC94 antibody (83580-1-RR, Clone: 240517A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83580-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 83580-1-RR (same clone as 83580-1-PBS)

    IF Staining of A549 using 83580-1-RR (same clone as 83580-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCDC94 antibody (83580-1-RR, Clone: 240517A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83580-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83580-1-RR (same clone as 83580-1-PBS)

    FC experiment of A431 using 83580-1-RR (same clone as 83580-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CCDC94 Recombinant antibody (83580-1-RR, Clone:240517A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83580-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00553-2

    Cytometric bead array standard curve of MP00553-2

    Cytometric bead array standard curve of MP00553-2, CCDC94 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83580-1-PBS. Detection antibody: 83580-2-PBS. Standard: Ag13764. Range: 0.78-100 ng/mL.

  • Cytometric bead array standard curve of MP00553-3

    Cytometric bead array standard curve of MP00553-3

    Cytometric bead array standard curve of MP00553-3, CCDC94 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83580-1-PBS. Detection antibody: 83580-3-PBS. Standard: Ag13764. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83580-1-RR

    Affinity and Kinetic Characterization of 83580-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83580-1-RR against Human CCDC94 were performed. The affinity constant is 1.08 nM.

CCDC94 Recombinant antibody, PBS Only (Capture)

CCDC94 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83580-1-PBS

产品说明书

CloneNo. 240517A1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

YJU2, Coiled-coil domain-containing protein 94, 240517A1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83580-1-PBS targets CCDC94 as part of a matched antibody pair:

MP00553-2: 83580-1-PBS capture and 83580-2-PBS detection (validated in Cytometric bead array)

MP00553-3: 83580-1-PBS capture and 83580-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 CCDC94 fusion protein Ag13764 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coiled-coil domain containing 94
别名 YJU2, Coiled-coil domain-containing protein 94, 240517A1
计算分子量 323 aa, 37 kDa
观测分子量40 kDa
GenBank蛋白编号BC000561
基因名称 CCDC94
Gene ID (NCBI) 55702
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BW85
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)