• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CCDC94 Recombinant antibody

CCDC94 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240517A1

Cat no : 83580-1-RR

Print datasheet

Synonyms

YJU2, Coiled-coil domain-containing protein 94, 240517A1

验证数据展示

  • WB analysis using 83580-1-RR

    WB analysis using 83580-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83580-1-RR (CCDC94 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 83580-1-RR

    IF Staining of A549 using 83580-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCDC94 antibody (83580-1-RR, Clone: 240517A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A549 using 83580-1-RR

    IF Staining of A549 using 83580-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCDC94 antibody (83580-1-RR, Clone: 240517A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 83580-1-RR

    FC experiment of A431 using 83580-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CCDC94 Recombinant antibody (83580-1-RR, Clone:240517A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83580-1-RR

    Affinity and Kinetic Characterization of 83580-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83580-1-RR against Human CCDC94 were performed. The affinity constant is 1.08 nM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cell, K-562 cells, HepG2 cells
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83580-1-RR targets CCDC94 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen CCDC94 fusion protein Ag13764 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name coiled-coil domain containing 94
Synonyms YJU2, Coiled-coil domain-containing protein 94, 240517A1
Calculated Molecular Weight 323 aa, 37 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC000561
Gene Symbol CCDC94
Gene ID (NCBI) 55702
RRIDAB_3671193
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BW85
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)

实验方案

Product Specific Protocols
WB protocol for CCDC94 antibody 83580-1-RRDownload protocol
IF protocol for CCDC94 antibody 83580-1-RRDownload protocol
FC protocol for CCDC94 antibody 83580-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols