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验证数据展示

  • WB analysis using 83606-5-RR

    WB analysis using 83606-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83606-5-RR (PIK3R2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 83606-5-RR

    IF Staining of Jurkat using 83606-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HeLa using 83606-5-RR

    IF Staining of HeLa using 83606-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

PI3 Kinase p85 Beta Recombinant antibody

PI3 Kinase p85 Beta Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83606-5-RR

产品说明书

CloneNo. 240531B5

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

PI3K p85 β, PI3K p85(beta), PI3K p85β, PIK3R2, p85β

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF/ICC detected inJurkat cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PIK3R2 Ready-To-Use IHC Kit. PIK3R2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83606-5-RR targets PI3 Kinase p85 Beta in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 PI3 Kinase p85 Beta fusion protein Ag14367 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphoinositide-3-kinase, regulatory subunit 2 (beta)
别名 PI3K p85 β, PI3K p85(beta), PI3K p85β, PIK3R2, p85β
计算分子量 728 aa, 82 kDa
观测分子量 82 kDa
GenBank蛋白编号BC014170
基因名称 PI3 Kinase p85 Beta
Gene ID (NCBI) 5296
RRIDAB_3671219
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO00459
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIK3R2 is also named as PI3 Kinase p85 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).

实验方案

Product Specific Protocols
WB protocol for PI3 Kinase p85 Beta antibody 83606-5-RRDownload protocol
IF protocol for PI3 Kinase p85 Beta antibody 83606-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols