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验证数据展示

  • WB analysis using 83606-5-RR (same clone as 83606-5-PBS)

    WB analysis using 83606-5-RR (same clone as 83606-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83606-5-RR (PIK3R2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83606-5-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 83606-5-RR (same clone as 83606-5-PBS)

    IF Staining of Jurkat using 83606-5-RR (same clone as 83606-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83606-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83606-5-RR (same clone as 83606-5-PBS)

    IF Staining of HeLa using 83606-5-RR (same clone as 83606-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83606-5-PBS in a different storage buffer formulation.

PI3 Kinase p85 Beta Recombinant antibody, PBS Only

PI3 Kinase p85 Beta Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83606-5-PBS

产品说明书

CloneNo. 240531B5

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

PI3K p85 β, PI3K p85(beta), PI3K p85β, PIK3R2, p85β

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PIK3R2 Ready-To-Use IHC Kit. PIK3R2 primary antibody included.

产品信息

83606-5-PBS targets PI3 Kinase p85 Beta in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 PI3 Kinase p85 Beta fusion protein Ag14367 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphoinositide-3-kinase, regulatory subunit 2 (beta)
别名 PI3K p85 β, PI3K p85(beta), PI3K p85β, PIK3R2, p85β
计算分子量 728 aa, 82 kDa
观测分子量 82 kDa
GenBank蛋白编号BC014170
基因名称 PI3 Kinase p85 Beta
Gene ID (NCBI) 5296
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO00459
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PIK3R2 is also named as PI3 Kinase p85 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).