PIK3R2 Recombinant antibody
PIK3R2 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240531B5
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells |
Planning an IHC experiment? We recommend our IHCeasy PIK3R2 Ready-To-Use IHC Kit. PIK3R2 primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83606-5-RR is PIK3R2 Fusion Protein expressed in E. coli.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human, rat |
Immunogen | PIK3R2 fusion protein Ag14367 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | phosphoinositide-3-kinase, regulatory subunit 2 (beta) |
Synonyms | p85, p85 BETA, P85B, PI3 kinase subunit p85 beta, PI3K regulatory subunit beta, PIK3R2 |
Calculated Molecular Weight | 728 aa, 82 kDa |
Observed Molecular Weight | 82 kDa |
GenBank Accession Number | BC014170 |
Gene Symbol | PIK3R2 |
Gene ID (NCBI) | 5296 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | O00459 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIK3R2 is also named as PI3 Kinase p85 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).
实验方案
Product Specific Protocols | |
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WB protocol for PIK3R2 antibody 83606-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |