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验证数据展示

  • WB analysis using 83621-6-RR

    WB analysis using 83621-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83621-6-RR (CDK8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • FC experiment of Caco-2 using 83621-6-RR

    FC experiment of Caco-2 using 83621-6-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug CDK8 Recombinant antibody (83621-6-RR, Clone:240598G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HeLa using 83621-6-RR

    IF Staining of HeLa using 83621-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK8 antibody (83621-6-RR, Clone: 240598G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83621-6-RR

    Affinity and Kinetic Characterization of 83621-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83621-6-RR against Human CDK8 were performed. The affinity constant is 12.5 nM.

View 3D epitope

CDK8 Recombinant antibody

CDK8 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83621-6-RR

产品说明书

CloneNo. 240598G9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

K35, EC:2.7.11.22, Cyclin-dependent kinase 8, Cyclin dependent kinase 8, Cell division protein kinase 8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488

规格: 
50 μL
100 μL
150 μL

¥2480/ 100 μL

浓度: 1000 ug/ml

库存：现货

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells, A549 cells, HCT 116 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inCaco-2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83621-6-RR targets CDK8 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 CDK8 fusion protein Ag17148 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 8
别名 K35, EC:2.7.11.22, Cyclin-dependent kinase 8, Cyclin dependent kinase 8, Cell division protein kinase 8
计算分子量 464 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC069634
基因名称 CDK8
Gene ID (NCBI) 1024
RRIDAB_3671234
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP49336
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CDK8 antibody 83621-6-RRDownload protocol
IF protocol for CDK8 antibody 83621-6-RRDownload protocol
FC protocol for CDK8 antibody 83621-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols