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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83621-6-RR (same clone as 83621-6-PBS)

    WB analysis using 83621-6-RR (same clone as 83621-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83621-6-RR (CDK8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83621-6-PBS in a different storage buffer formulation.

  • FC experiment of Caco-2 using 83621-6-RR (same clone as 83621-6-PBS)

    FC experiment of Caco-2 using 83621-6-RR (same clone as 83621-6-PBS)

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug CDK8 Recombinant antibody (83621-6-RR, Clone:240598G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83621-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83621-6-RR (same clone as 83621-6-PBS)

    IF Staining of HeLa using 83621-6-RR (same clone as 83621-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK8 antibody (83621-6-RR, Clone: 240598G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83621-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83621-6-RR

    Affinity and Kinetic Characterization of 83621-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83621-6-RR against Human CDK8 were performed. The affinity constant is 12.5 nM.

CDK8 Recombinant antibody, PBS Only

CDK8 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83621-6-PBS

产品说明书

CloneNo. 240598G9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

K35, EC:2.7.11.22, Cyclin-dependent kinase 8, Cyclin dependent kinase 8, Cell division protein kinase 8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83621-6-PBS targets CDK8 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 CDK8 fusion protein Ag17148 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 8
别名 K35, EC:2.7.11.22, Cyclin-dependent kinase 8, Cyclin dependent kinase 8, Cell division protein kinase 8
计算分子量 464 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC069634
基因名称 CDK8
Gene ID (NCBI) 1024
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP49336
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C