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验证数据展示

  • WB analysis using 83638-3-RR

    WB analysis using 83638-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83638-3-RR (RCN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83638-3-RR

    WB analysis using 83638-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83638-3-RR (RCN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 83638-3-RR

    IF Staining of U2OS using 83638-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RCN1 antibody (83638-3-RR, Clone: 240473E9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83638-3-RR

    FC experiment of HeLa using 83638-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RCN1 Recombinant antibody (83638-3-RR, Clone:240473E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83638-3-RR

    Affinity and Kinetic Characterization of 83638-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83638-3-RR against Human RCN1 were performed. The affinity constant is below 1 pM.

RCN1 Recombinant antibody

RCN1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83638-3-RR

产品说明书

CloneNo. 240473E9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Reticulocalbin 1, Reticulocalbin-1, RCN, RCAL, PIG20

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A375 cells, SH-SY5Y cells, HuH-7 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83638-3-RR targets RCN1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 RCN1 fusion protein Ag13256 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 reticulocalbin 1, EF-hand calcium binding domain
别名 Reticulocalbin 1, Reticulocalbin-1, RCN, RCAL, PIG20
计算分子量 39 kDa
观测分子量 44-46 kDa
GenBank蛋白编号BC010120
基因名称 RCN1
Gene ID (NCBI) 5954
RRIDAB_3671250
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15293
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Reticulocalbin-1 (RCN1) belongs to the CREC family. Reticulocalbin‐1, an endoplasmic reticulum protein, plays a crucial role in calcium homeostasis and inhibits ER stress‐induced apoptosis (PMID: 28319095). Its main functions include maintaining intracellular homeostasis and regulating cell proliferation and apoptosis, and it plays an important role in the development of various tumours (PMID: 38057406). RCN1 is expressed aberrantly and at a high level in various tumors, including acute myeloid leukemia (AML) (PMID: 37746742). In multiple cancers, such as glioblastoma, non‐small cell lung cancer, renal cell carcinoma, hepatocellular carcinoma, and oral squamous cell carcinoma, the overexpression of RCN1 has been observed indicating its involvement in tumorigenesis and invasion (PMID: 30172915, PMID: 34722631).

实验方案

Product Specific Protocols
WB protocol for RCN1 antibody 83638-3-RRDownload protocol
IF protocol for RCN1 antibody 83638-3-RRDownload protocol
FC protocol for RCN1 antibody 83638-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols