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TADA1L Recombinant antibody

TADA1L Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240637F7

Cat no : 83641-4-RR

Print datasheet

Synonyms

STAF42, SPT3-associated factor 42, SPT3 associated factor 42, RP1 9E21.4, KIAA0764

验证数据展示

  • WB analysis using 83641-4-RR

    WB analysis using 83641-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 83641-4-RR

    WB analysis of human testis using 83641-4-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83641-4-RR

    FC experiment of HepG2 using 83641-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Tada1l Recombinant Antibody (83641-4-RR, Clone:240637F7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83641-4-RR

    Affinity and Kinetic Characterization of 83641-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83641-4-RR against Human TADA1L were performed. The affinity constant is 0.43 nM.

经过测试的应用

Positive WB detected inMCF-7 cells, human testis tissue, HepG2 cells, mouse testis tissue, rat testis tissue
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83641-4-RR is TADA1L Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TADA1L fusion protein Ag14118 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transcriptional adaptor 1 (HFI1 homolog, yeast)-like
Synonyms STAF42, SPT3-associated factor 42, SPT3 associated factor 42, RP1 9E21.4, KIAA0764
Calculated Molecular Weight 335 aa, 37 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC015401
Gene Symbol TADA1L
Gene ID (NCBI) 117143
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96BN2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The function of TADA1L has not been widely studied, and is yet to be fully elucidated. It probably involved in transcriptional regulation.

实验方案

Product Specific Protocols
WB protocol for TADA1L antibody 83641-4-RRDownload protocol
FC protocol for TADA1L antibody 83641-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols