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验证数据展示

  • WB analysis using 83641-4-RR (same clone as 83641-4-PBS)

    WB analysis using 83641-4-RR (same clone as 83641-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83641-4-PBS in a different storage buffer formulation.

  • WB analysis of human testis using 83641-4-RR (same clone as 83641-4-PBS)

    WB analysis of human testis using 83641-4-RR (same clone as 83641-4-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83641-4-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83641-4-RR (same clone as 83641-4-PBS)

    FC experiment of HepG2 using 83641-4-RR (same clone as 83641-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Tada1l Recombinant Antibody (83641-4-RR, Clone:240637F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83641-4-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 83641-4-RR (same clone as 83641-4-PBS)

    WB analysis of MCF-7 using 83641-4-RR (same clone as 83641-4-PBS)

    WB result of TADA1L antibody (83641-4-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TADA1L transfected MCF-7 cells. This data was developed using the same antibody clone with 83641-4-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 83641-4-RR (same clone as 83641-4-PBS)

    IF Staining of MCF-7 using 83641-4-RR (same clone as 83641-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TADA1L antibody (83641-4-RR, Clone: 240637F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83641-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00624-1

    Cytometric bead array standard curve of MP00624-1

    Cytometric bead array standard curve of MP00624-1, TADA1L Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83641-4-PBS. Detection antibody: 83641-5-PBS. Standard: Ag14118. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83641-4-RR

    Affinity and Kinetic Characterization of 83641-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83641-4-RR against Human TADA1L were performed. The affinity constant is 0.43 nM.

  • Featured Product
  • KD/KO验证

TADA1L Recombinant monoclonal antibody, PBS Only (Capture)

TADA1L Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83641-4-PBS

产品说明书

CloneNo. 240637F7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

240637F7, KIAA0764, RP1 9E21.4, SPT3 associated factor 42, SPT3-associated factor 42

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83641-4-PBS targets TADA1L as part of a matched antibody pair:

MP00624-1: 83641-4-PBS capture and 83641-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14118

Product name: Recombinant human TADA1L protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-335 aa of BC015401

Sequence: MATFVSELEAAKKNLSEALGDNVKQYWANLKLWFKQKISKEEFDLEAHRLLTQDNVHSHNDFLLAILTRCQILVSTPDGAGSLPWPGGSAAKPGKPKGKKKLSSVRQKFDHRFQPQNPLSGAQQFVAKDPQDDDDLKLCSHTMMLPTRGQLEGRMIVTAYEHGLDNVTEEAVSAVVYAVENHLKDILTSVVSRRKAYRLRDGHFKYAFGSNVTPQPYLKNSVVAYNNLIESPPAFTAPCAGQNPASHPPPDDAEQQAALLLACSGDTLPASLPPVNMYDLFEALQVHREVIPTHTVYALNIERIITKLWHPNHEELQQDKVHRQRLAAKEGLLLC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcriptional adaptor 1 (HFI1 homolog, yeast)-like
别名 240637F7, KIAA0764, RP1 9E21.4, SPT3 associated factor 42, SPT3-associated factor 42
计算分子量 335 aa, 37 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC015401
基因名称 TADA1L
Gene ID (NCBI) 117143
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96BN2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The function of TADA1L has not been widely studied, and is yet to be fully elucidated. It probably involved in transcriptional regulation.

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