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HSP47/SERPINH1 Recombinant antibody, PBS Only (Detector)

HSP47/SERPINH1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240752F6

Cat no : 83689-3-PBS

Print datasheet

Synonyms

HSP47, SERPINH1, CBP2, CBP1, AsTP3

验证数据展示

  • WB analysis using 83689-3-RR (same clone as 83689-3-PBS)

    WB analysis using 83689-3-RR (same clone as 83689-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83689-3-RR (HSP47/SERPINH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83689-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83689-3-RR (same clone as 83689-3-PBS)

    IHC staining of human ovarian cancer using 83689-3-RR (same clone as 83689-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83689-3-RR (HSP47/SERPINH1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83689-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP47 antibody included
  • FC experiment of HepG2 using 83689-3-RR (same clone as 83689-3-PBS)

    FC experiment of HepG2 using 83689-3-RR (same clone as 83689-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HSP47/SERPINH1 Recombinant antibody (83689-3-RR, Clone:240752F6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83689-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00677-2

    Sandwich ELISA standard curve of MP00677-2

    Sandwich ELISA standard curve of MP00677-2, Human HSP47/SERPINH1 Recombinant Matched Antibody Pair - PBS only. 83689-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1201. 83689-3-PBS was HRP conjugated as the detection antibody. Range: 0.23-15 ng/mL

  • Sample test of MP00677-2

    Sample test of MP00677-2

    HepG2, Hela and MCF-7 cells were cultured in DMEM supplemented with 10% fetal bonive serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Aliquots of the cell culture supernates were removed. Cells were lysed and assayed for human HSP47/SERPINH1. Human HSP47/SERPINH1 in HepG2 cell lysate measured 17.9 ng/mL. Human HSP47/SERPINH1 in Hela cell lysate measured 56.9 ng/mL. Human HSP47/SERPINH1 in MCF-7 cell lysate measured 35.1 ng/mL.

  • Affinity and Kinetic Characterization of 83689-3-RR

    Affinity and Kinetic Characterization of 83689-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83689-3-RR against Human HSP47/SERPINH1 were performed. The affinity constant is 12.9 nM.

Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.

产品信息

83689-3-PBS targets HSP47/SERPINH1 as part of a matched antibody pair:

MP00677-2: 83689-4-PBS capture and 83689-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Synonyms HSP47, SERPINH1, CBP2, CBP1, AsTP3
Calculated Molecular Weight46kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberNM_001235.5
Gene Symbol HSP47/SERPINH1
Gene ID (NCBI) 871
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP50454
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.