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验证数据展示

  • WB analysis using 83690-4-RR

    WB analysis using 83690-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83690-4-RR (MARS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83690-4-RR

    IF Staining of HepG2 using 83690-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (83690-4-RR, Clone: 240648G4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human stomach cancer using 83690-4-RR

    IHC staining of human stomach cancer using 83690-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83690-4-RR (MARS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with MARS1 antibody included
  • IF Staining of HepG2 using 83690-4-RR

    IF Staining of HepG2 using 83690-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (83690-4-RR, Clone: 240648G4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83690-4-RR

    Affinity and Kinetic Characterization of 83690-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83690-4-RR against Human MARS were performed. The affinity constant is 1.90 nM.

MARS Recombinant antibody

MARS Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83690-4-RR

产品说明书

CloneNo. 240648G4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

MARS1, METRS, Methionyl-tRNA synthetase, Methionine--tRNA ligase, cytoplasmic, EC:6.1.1.10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, A549 cells, PC-12 cells, mouse testis tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MARS1 Ready-To-Use IHC Kit. MARS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83690-4-RR targets MARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MARS fusion protein Ag6619 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methionyl-tRNA synthetase
别名 MARS1, METRS, Methionyl-tRNA synthetase, Methionine--tRNA ligase, cytoplasmic, EC:6.1.1.10
计算分子量 101 kDa
观测分子量101 kDa
GenBank蛋白编号BC002384
基因名称 MARS
Gene ID (NCBI) 4141
RRIDAB_3671293
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP56192
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The methionyl-tRNA synthetase (MARS) gene encodes cytoplasmic methionyl-tRNA synthetase (MetRS) responsible for catalyzing the ligation of methionine to tRNA. MetRS belongs to a family of aminoacyl-tRNA synthetases that play critical roles in protein biosynthesis by charging tRNAs with their cognate amino acids. MetRS overexpression was shown to be evident in human colon cancer patients. MetRS may thus be involved in oncogenic transformation. MetRS has been proven as a potential prognostic marker candidate for the clinical prognostic prediction of non-small-cell lung cancer (NSCLC) in patients. MetRS can also be used to detect intracellular oxidative stress and control protein synthesis under oxidative stress (PMID: 30271085, PMID: 34679529, PMID: 32404475). The calculated molecular weight of MetRS is 101 kDa.

实验方案

Product Specific Protocols
WB protocol for MARS antibody 83690-4-RRDownload protocol
IHC protocol for MARS antibody 83690-4-RRDownload protocol
IF protocol for MARS antibody 83690-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols