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验证数据展示

  • WB analysis using 83690-4-RR (same clone as 83690-4-PBS)

    WB analysis using 83690-4-RR (same clone as 83690-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83690-4-RR (MARS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83690-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83690-4-RR (same clone as 83690-4-PBS)

    IF Staining of HepG2 using 83690-4-RR (same clone as 83690-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (83690-4-RR, Clone: 240648G4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83690-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00679-1

    Sandwich ELISA standard curve of MP00679-1

    Sandwich ELISA standard curve of MP00679-1, Human MARS Recombinant Matched Antibody Pair - PBS only. 83690-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6619. 83690-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP00679-1

    Sample test of MP00679-1

    HepG2 and A549 cell lysates were measured. The human MARS concentration of detected samples was determined to be 139.60 ng/mL (based on a 3.00 mg/mL extract load) in HepG2 cell lysate and 85.10 ng/mL (based on a 3.00 mg/mL extract load) in A549 cell lysate.

  • IHC staining of human stomach cancer using 83690-4-RR (same clone as 83690-4-PBS)

    IHC staining of human stomach cancer using 83690-4-RR (same clone as 83690-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83690-4-RR (MARS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83690-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MARS1 antibody included
  • IF Staining of HepG2 using 83690-4-RR (same clone as 83690-4-PBS)

    IF Staining of HepG2 using 83690-4-RR (same clone as 83690-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (83690-4-RR, Clone: 240648G4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83690-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00679-1

    Cytometric bead array standard curve of MP00679-1

    Cytometric bead array standard curve of MP00679-1, MARS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83690-4-PBS. Detection antibody: 83690-3-PBS. Standard: Ag6619. Range: 1.56-100 ng/mL.

  • Cytometric bead array standard curve of MP00679-2

    Cytometric bead array standard curve of MP00679-2

    Cytometric bead array standard curve of MP00679-2, MARS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83690-4-PBS. Detection antibody: 83690-2-PBS. Standard: Ag6619. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83690-4-RR

    Affinity and Kinetic Characterization of 83690-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83690-4-RR against Human MARS were performed. The affinity constant is 1.90 nM.

MARS Recombinant antibody, PBS Only (Capture)

MARS Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83690-4-PBS

产品说明书

CloneNo. 240648G4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

MARS1, METRS, Methionyl-tRNA synthetase, Methionine--tRNA ligase, cytoplasmic, EC:6.1.1.10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MARS1 Ready-To-Use IHC Kit. MARS1 primary antibody included.

产品信息

83690-4-PBS targets MARS as part of a matched antibody pair:

MP00679-1: 83690-4-PBS capture and 83690-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00679-2: 83690-4-PBS capture and 83690-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 MARS fusion protein Ag6619 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methionyl-tRNA synthetase
别名 MARS1, METRS, Methionyl-tRNA synthetase, Methionine--tRNA ligase, cytoplasmic, EC:6.1.1.10
计算分子量 101 kDa
观测分子量101 kDa
GenBank蛋白编号BC002384
基因名称 MARS
Gene ID (NCBI) 4141
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP56192
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The methionyl-tRNA synthetase (MARS) gene encodes cytoplasmic methionyl-tRNA synthetase (MetRS) responsible for catalyzing the ligation of methionine to tRNA. MetRS belongs to a family of aminoacyl-tRNA synthetases that play critical roles in protein biosynthesis by charging tRNAs with their cognate amino acids. MetRS overexpression was shown to be evident in human colon cancer patients. MetRS may thus be involved in oncogenic transformation. MetRS has been proven as a potential prognostic marker candidate for the clinical prognostic prediction of non-small-cell lung cancer (NSCLC) in patients. MetRS can also be used to detect intracellular oxidative stress and control protein synthesis under oxidative stress (PMID: 30271085, PMID: 34679529, PMID: 32404475). The calculated molecular weight of MetRS is 101 kDa.