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MARS Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human, mouse, rat

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 1.56-100 ng/mL

Sandwich ELISA: 39.1-2500 pg/mL

Cat No : MP00679-1

Print datasheet

Synonyms

FLJ35667, MARS, Methionine tRNA ligase, methionyl tRNA synthetase, METRS, MTRNS

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00679-1, Human MARS Recombinant Matched Antibody Pair - PBS only. 83690-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6619. 83690-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

    Sandwich ELISA standard curve of MP00679-1

    Sandwich ELISA standard curve of MP00679-1, Human MARS Recombinant Matched Antibody Pair - PBS only. 83690-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6619. 83690-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • HepG2 and A549 cell lysates were measured. The human MARS concentration of detected samples was determined to be 139.60 ng/mL (based on a 3.00 mg/mL extract load) in HepG2 cell lysate and 85.10 ng/mL (based on a 3.00 mg/mL extract load) in A549 cell lysate.

    Sample test of MP00679-1

    HepG2 and A549 cell lysates were measured. The human MARS concentration of detected samples was determined to be 139.60 ng/mL (based on a 3.00 mg/mL extract load) in HepG2 cell lysate and 85.10 ng/mL (based on a 3.00 mg/mL extract load) in A549 cell lysate.

  • Cytometric bead array standard curve of MP00679-1, MARS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83690-4-PBS. Detection antibody: 83690-3-PBS. Standard: Ag6619. Range: 1.56-100 ng/mL.

    Cytometric bead array standard curve of MP00679-1

    Cytometric bead array standard curve of MP00679-1, MARS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83690-4-PBS. Detection antibody: 83690-3-PBS. Standard: Ag6619. Range: 1.56-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00679-1 targets MARS in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 1.56-100 ng/mL (Cytometric Bead Array); 39.1-2500 pg/mL (Sandwich ELISA);

Capture antibody: MARS Recombinant antibody, PBS Only (Capture) 83690-4-PBS (240648G4). 100 μg. Concentration 1 mgl/ml.

Detection antibody: MARS Recombinant antibody, PBS Only (Detector) 83690-3-PBS (240648F10). 100 μg. Concentration 1 mgl/ml.

Alternative MARS matched antibody pairs: MP00679-2, MP00679-3

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83690-4-PBS
Tested Reactivity human, mouse, rat
Immunogen MARS fusion protein Ag6619
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name methionyl-tRNA synthetase
Synonyms MARS1, METRS, Methionyl-tRNA synthetase, Methionine--tRNA ligase, cytoplasmic, EC:6.1.1.10
GenBank accession numberBC002384
Gene symbol MARS
UniprotP56192
Gene ID (NCBI) 4141
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83690-3-PBS
Tested Reactivity human
Immunogen MARS fusion protein Ag6619
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name methionyl-tRNA synthetase
Synonyms MARS1, METRS, Methionyl-tRNA synthetase, Methionine--tRNA ligase, cytoplasmic, EC:6.1.1.10
GenBank accession numberBC002384
Gene symbol MARS
UniprotP56192
Gene ID (NCBI) 4141
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C