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MARS Recombinant antibody

MARS Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240648G4

Cat no : 83690-4-RR

Print datasheet

Synonyms

MARS1, METRS, Methionyl-tRNA synthetase, Methionine--tRNA ligase, cytoplasmic, EC:6.1.1.10

验证数据展示

  • WB analysis using 83690-4-RR

    WB analysis using 83690-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83690-4-RR (MARS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83690-4-RR

    IF Staining of HepG2 using 83690-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (83690-4-RR, Clone: 240648G4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human stomach cancer using 83690-4-RR

    IHC staining of human stomach cancer using 83690-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83690-4-RR (MARS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with MARS1 antibody included
  • IF Staining of HepG2 using 83690-4-RR

    IF Staining of HepG2 using 83690-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (83690-4-RR, Clone: 240648G4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83690-4-RR

    Affinity and Kinetic Characterization of 83690-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83690-4-RR against Human MARS were performed. The affinity constant is 1.90 nM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, A549 cells, PC-12 cells, mouse testis tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MARS1 Ready-To-Use IHC Kit. MARS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83690-4-RR targets MARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MARS fusion protein Ag6619 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name methionyl-tRNA synthetase
Synonyms MARS1, METRS, Methionyl-tRNA synthetase, Methionine--tRNA ligase, cytoplasmic, EC:6.1.1.10
Calculated Molecular Weight 101 kDa
Observed Molecular Weight101 kDa
GenBank Accession NumberBC002384
Gene Symbol MARS
Gene ID (NCBI) 4141
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56192
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The methionyl-tRNA synthetase (MARS) gene encodes cytoplasmic methionyl-tRNA synthetase (MetRS) responsible for catalyzing the ligation of methionine to tRNA. MetRS belongs to a family of aminoacyl-tRNA synthetases that play critical roles in protein biosynthesis by charging tRNAs with their cognate amino acids. MetRS overexpression was shown to be evident in human colon cancer patients. MetRS may thus be involved in oncogenic transformation. MetRS has been proven as a potential prognostic marker candidate for the clinical prognostic prediction of non-small-cell lung cancer (NSCLC) in patients. MetRS can also be used to detect intracellular oxidative stress and control protein synthesis under oxidative stress (PMID: 30271085, PMID: 34679529, PMID: 32404475). The calculated molecular weight of MetRS is 101 kDa.

实验方案

Product Specific Protocols
WB protocol for MARS antibody 83690-4-RRDownload protocol
IHC protocol for MARS antibody 83690-4-RRDownload protocol
IF protocol for MARS antibody 83690-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols