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GBP4 Recombinant antibody

GBP4 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240730H5

Cat No : 83699-4-RR

Print datasheet

Synonyms

Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4, EC:3.6.5.-

验证数据展示

  • FC experiment of HepG2 using 83699-4-RR

    FC experiment of HepG2 using 83699-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GBP4 Recombinant antibody (83699-4-RR, Clone:240730H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HeLa using 83699-4-RR

    WB analysis of HeLa using 83699-4-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 83699-4-RR (GBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83699-4-RR

    Affinity and Kinetic Characterization of 83699-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83699-4-RR against Human GBP4 were performed. The affinity constant is 11.6 pM.

经过测试的应用

Positive WB detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83699-4-RR is GBP4 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen GBP4 fusion protein Ag12006 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name guanylate binding protein 4
Synonyms Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4, EC:3.6.5.-
Calculated Molecular Weight 640 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC070055
Gene Symbol GBP4
Gene ID (NCBI) 115361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96PP9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate-binding protein 4 (GBP4) is an IFN-inducible GTPase that belongs to the GB1/RHD3 GTPase family. GBP4 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. GBP4 negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor. The molecular weight of GBP4 is 73 kDa.

实验方案

Product Specific Protocols
WB protocol for GBP4 antibody 83699-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols