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GBP4 Recombinant antibody, PBS Only

GBP4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240730H5

Cat no : 83699-4-PBS

Print datasheet

Synonyms

Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4, EC:3.6.5.-

验证数据展示

  • FC experiment of HepG2 using 83699-4-RR (same clone as 83699-4-PBS)

    FC experiment of HepG2 using 83699-4-RR (same clone as 83699-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GBP4 Recombinant antibody (83699-4-RR, Clone:240730H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83699-4-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 83699-4-RR (same clone as 83699-4-PBS)

    WB analysis of HeLa using 83699-4-RR (same clone as 83699-4-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 83699-4-RR (GBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83699-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83699-4-RR

    Affinity and Kinetic Characterization of 83699-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83699-4-RR against Human GBP4 were performed. The affinity constant is 11.6 pM.

产品信息

83699-4-PBS targets GBP4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen GBP4 fusion protein Ag12006 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name guanylate binding protein 4
Synonyms Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4, EC:3.6.5.-
Calculated Molecular Weight 640 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC070055
Gene Symbol GBP4
Gene ID (NCBI) 115361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96PP9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Guanylate-binding protein 4 (GBP4) is an IFN-inducible GTPase that belongs to the GB1/RHD3 GTPase family. GBP4 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. GBP4 negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor. The molecular weight of GBP4 is 73 kDa.