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验证数据展示

  • WB analysis using 83713-2-RR (same clone as 83713-2-PBS)

    WB analysis using 83713-2-RR (same clone as 83713-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83713-2-RR (CD34 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83713-2-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 83713-2-RR (same clone as 83713-2-PBS)

    IF Staining of NIH/3T3 using 83713-2-RR (same clone as 83713-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CD34 antibody (83713-2-RR, Clone: 240659C7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83713-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 83713-2-RR (same clone as 83713-2-PBS)

    IHC staining of mouse testis using 83713-2-RR (same clone as 83713-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83713-2-RR (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83713-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00684-1

    Cytometric bead array standard curve of MP00684-1

    Cytometric bead array standard curve of MP00684-1, MOUSE CD34 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83713-2-PBS. Detection antibody: 83713-4-PBS. Standard: Eg0854. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 83713-2-RR

    Affinity and Kinetic Characterization of 83713-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83713-2-RR against Mouse CD34 were performed. The affinity constant is 1.09 nM.

Mouse CD34 Recombinant antibody, PBS Only (Capture)

CD34 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83713-2-PBS

产品说明书

CloneNo. 240659C7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

CD34 antigen, 240659C7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83713-2-PBS targets CD34 as part of a matched antibody pair:

MP00684-1: 83713-2-PBS capture and 83713-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD34 antigen
别名 CD34 antigen, 240659C7
计算分子量41 kDa
观测分子量105 kDa
GenBank蛋白编号NM_133654
基因名称 CD34
Gene ID (NCBI) 12490
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ64314
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.