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验证数据展示

  • WB analysis using 83715-1-RR

    WB analysis using 83715-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83715-1-RR (Bif-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83715-1-RR

    IF Staining of HeLa using 83715-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bif-1 antibody (83715-1-RR, Clone: 240623E1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83715-1-RR

    Affinity and Kinetic Characterization of 83715-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83715-1-RR against Human Bif-1 were performed. The affinity constant is 0.71 nM.

Bif-1 Recombinant antibody

Bif-1 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83715-1-RR

产品说明书

CloneNo. 240623E1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

SH3GLB1, Endophilin-B1, Endophilin B1, Bif1, Bax-interacting factor 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Bif-1 Ready-To-Use IHC Kit. Bif-1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83715-1-RR targets Bif-1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Bif-1 fusion protein Ag7710 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SH3-domain GRB2-like endophilin B1
别名 SH3GLB1, Endophilin-B1, Endophilin B1, Bif1, Bax-interacting factor 1
计算分子量 41 kDa
观测分子量 41 kDa
GenBank蛋白编号BC007455
基因名称 Bif-1
Gene ID (NCBI) 51100
RRIDAB_3671317
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y371
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH3GLB1 also known as Bif-1, contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain, but shares no significant homology with members of the Bcl-2 family. SH3GLB1 is involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588).

实验方案

Product Specific Protocols
WB protocol for Bif-1 antibody 83715-1-RRDownload protocol
IF protocol for Bif-1 antibody 83715-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols