验证数据展示

Sandwich ELISA standard curve of MP00686-1
Sandwich ELISA standard curve of MP00686-1, Human MICA Recombinant Matched Antibody Pair - PBS only. 83725-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1176. 83725-1-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 pg/mL.
Sample test of MP00686-1
HUVEC cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MICA, and measured 70.4 pg/mL.
HeLa cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MICA, and measured 285.9 pg/mL.
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HUVEC cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MICA, and measured 70.4 pg/mL.
HeLa cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MICA, and measured 285.9 pg/mL.
Cytometric bead array standard curve of MP00686-1
Cytometric bead array standard curve of MP00686-1, MICA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83725-3-PBS. Detection antibody: 83725-1-PBS. Standard: Eg1176. Range: 0.625-80 ng/mL.
Affinity and Kinetic Characterization of 83725-1-PBS
Biolayer interferometry (BLl) kinetic assays of 83725-1-PBS against Human MICA were performed. The affinity constant is 0.927 nM.

MICA Recombinant monoclonal antibody, PBS Only (Detector)

MICA Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83725-1-PBS

产品说明书

CloneNo. 240735D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

MIC A, MIC-A

缓冲液配方: PBS Only

PBS Only

偶联物: Unconjugated

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概述

经过测试的应用
产品信息

产品说明书

MSDS

产品信息

83725-1-PBS targets MICA as part of a matched antibody pair:

MP00686-1: 83725-3-PBS capture and 83725-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用	Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性	human
免疫原	CatNo: Eg1176 Product name: Recombinant Human MICA protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 24-308 aa of / Sequence: EPHSLRYNLTVLSWDGSVQSGFLTEVHLDGQPFLRCDRQKCRAKPQGQWAEDVLGNKTWDRETRDLTGNGKDLRMTLAHIKDQKEGLHSLQEIRVCEIHEDNSTRSSQHFYYDGELFLSQNLETEEWTMPQSSRAQTLAMNVRNFLKEDAMKTKTHYHAMHADCLQELRRYLKSGVVLRRTVPPMVNVTRSEASEGNITVTCRASGFYPWNITLSWRQDGVSLSHDTQQWGDVLPDGNGTYQTWVATRICQGEEQRFTCYMEHSGNHSTHPVPSGKVLVLQSHWQ 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Recombinant
产品类型	Antibody
全称	MHC class I polypeptide-related sequence A
别名	MIC A, MIC-A
计算分子量	43 kDa
GenBank蛋白编号	AAH16929.1
基因名称	MICA
Gene ID (NCBI)	100507436
偶联类型	Unconjugated
形式	Liquid
纯化方式	Protein A purification
UNIPROT ID	Q29983
储存缓冲液	PBS only, pH 7.3.
储存条件	Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

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验证数据展示

Sandwich ELISA standard curve of MP00686-1

Sample test of MP00686-1

Cytometric bead array standard curve of MP00686-1

Affinity and Kinetic Characterization of 83725-1-PBS

MICA Recombinant monoclonal antibody, PBS Only (Detector)

MICA Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

产品信息

验证数据展示

Sandwich ELISA standard curve of MP00686-1

Sample test of MP00686-1

Cytometric bead array standard curve of MP00686-1

Affinity and Kinetic Characterization of 83725-1-PBS

MICA Recombinant monoclonal antibody, PBS Only (Detector)

MICA Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

产品信息

MICA Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test