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验证数据展示

  • IF Staining of HeLa using 83760-3-RR (same clone as 83760-3-PBS)

    IF Staining of HeLa using 83760-3-RR (same clone as 83760-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCL9 antibody (83760-3-RR, Clone: 240682A9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83760-3-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 83760-3-RR (same clone as 83760-3-PBS)

    FC experiment of SH-SY5Y using 83760-3-RR (same clone as 83760-3-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Bcl9 Recombinant Antibody (83760-3-RR, Clone:240682A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83760-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00739-2

    Cytometric bead array standard curve of MP00739-2

    Cytometric bead array standard curve of MP00739-2, BCL9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83760-3-PBS. Detection antibody: 83760-5-PBS. Standard: Ag19158. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00739-3

    Cytometric bead array standard curve of MP00739-3

    Cytometric bead array standard curve of MP00739-3, BCL9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83760-3-PBS. Detection antibody: 83760-4-PBS. Standard: Ag19158. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83760-3-PBS

    Affinity and Kinetic Characterization of 83760-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83760-3-PBS against Human BCL9 was performed. The affinity constant is 5.40 nM.

  • Affinity and Kinetic Characterization of 83760-3-RR

    Affinity and Kinetic Characterization of 83760-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83760-3-RR against Human BCL9 were performed. The affinity constant is 5.39 nM.

BCL9 Recombinant antibody, PBS Only (Capture)

BCL9 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83760-3-PBS

产品说明书

CloneNo. 240682A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

Bcl-9, Bcl 9, B-cell lymphoma 9 protein, B-cell CLL/lymphoma 9 protein, B cell CLL/lymphoma 9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83760-3-PBS targets BCL9 as part of a matched antibody pair:

MP00739-2: 83760-3-PBS capture and 83760-5-PBS detection (validated in Cytometric bead array)

MP00739-3: 83760-3-PBS capture and 83760-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 BCL9 fusion protein Ag19158 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell CLL/lymphoma 9
别名 Bcl-9, Bcl 9, B-cell lymphoma 9 protein, B-cell CLL/lymphoma 9 protein, B cell CLL/lymphoma 9
计算分子量 1426 aa, 149 kDa
GenBank蛋白编号BC116451
基因名称 BCL9
Gene ID (NCBI) 607
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00512
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C